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KMID : 0613820070170020298
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Journal of Life Science
2007 Volume.17 No. 2 p.298 ~ p.304
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Identification and Characterization of Stationary-phase Specific Cytosolic Protein in Salmonella typhimurium
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Yoo Ah-Young
Kim Young-Hee
Yu Jong-Earn
Kim Sam-Woong
Baik Hyung-Suk
Kang Ho-Young
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Abstract
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Salmonella is facultative intracellular pathogen that can survive and replicate in macrophages even though these cells are equipped with a plethora of anti-microbial mechanisms. To survive in this hostile intracellular environment, Salmonella has evolved numerous defense mechanisms. An approximately 20 kDa protein was detected as a stationary-phase specific protein band in cytosolic fraction. It was identified as a DNA binding protein in stationary phase (Dps) by analysis of MALDI-TOF assay. It has been known that Dps, the protein produced in the stationary phase of bacteria, allows DNA to form chromatin by binding to DNA nonspecifically and protects DNA from reactive oxidative species (ROS). For further study, Dps specific polyclonal antibodies were generated by injection of purified Dps protein into rabbit. To examine the Finfluence of several regulatory proteins in the expression dps gene, Dps protein level in various S. typhimurium mutants defecting regulatory proteins were investigated by Westernblot using Dps specific polyclonal antibodies.
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KEYWORD
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Salmonella typhimurium, stationary phase, Dps
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